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Division of Consolidated Laboratory Services

BACTERIOLOGY REFERENCE CULTURE

Laboratory: Microbial Reference

Test Description: Identification of unusual aerobic and anaerobic bacteria isolated from clinical specimens.

Order Approval Required? No

Specimen Type: Bacterial Isolate Slant or Plate (Clinical Specimens)

Collection Container: Tryptic Soy Agar (TSA) Slant or other media appropriate for bacterial growth.

Optimal Volume: Not Applicable

Collection Guidelines: Not Applicable

Storage Container: Not Applicable

Storage Instructions: Room Temperature

Transportation Instructions: Room Temperature

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information:

Causes For Rejection:

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Media that has expired prior to inoculation.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results: Full isolate identification. Isolate may be forwarded to the Centers for Disease Control and Prevention (CDC) for further testing if necessary.

Test Description: Serotyping/grouping of Neisseria meningitidis and Haemophilus influenzae from sterile sources.

Order Approval Required? No

Collection Container: Chocolate Agar Slant or other media appropriate for Neisseria or Haemophilus growth.

Optimal Volume: Not Applicable

Collection Guidelines: Not Applicable

Storage Container: Not Applicable

Storage Instructions: Room Temperature

Transportation Instructions: Room Temperature

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information:

Causes For Rejection:

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Media that has expired prior to inoculation.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results: Full isolate identification including serotype. Isolate may be forwarded to the Centers for Disease Control and Prevention (CDC) for further testing if necessary.

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? No

Collection Container: Isolator Tube of Blood or Culture Broth Bottle

Optimal Volume: >3ml for adult; >1ml for pediatric

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible so that sample is processed no longer than 48 hours after collection.

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Room Temperature

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Due to agonal invasion of sterile tissues with skin, fecal or normal respiratory flora, the OCME may be contacted to direct identification of isolates. Patient history information provided by the OCME assists the laboratory in determining or prioritizing the types of organisms to target for identification that are consistent with this history as opposed to organisms that may otherwise be considered normal flora.

Causes For Rejection: Any sample rejections must be approved by the Group Manager, Director of Laboratory Operations, or Laboratory Director after consultation with the OCME

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results:

  • Quantification of organisms.
  • Organisms reported by cellular morphology.
  • Full identification will be performed for significant pathogens, pure culture isolates or a predominating organism in a mixed culture, and at the request of the OCME.

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? No

Collection Container: Sterile Tube

Optimal Volume: Minimum 1ml

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible so that sample is processed no longer than 48 hours after collection.

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Cerebrospinal fluid (CSF) for bacterial culture should be transported at room temperature. CSF for viral culture should be transported with cold packs. If a CSF specimen requires both bacterial culture and viral culture, but cannot be split into 2 tubes, the CSF should be transported with cold packs.

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Due to agonal invasion of sterile tissues with skin, fecal or normal respiratory flora, the OCME may be contacted to direct identification of isolates. Patient history information provided by the OCME assists the laboratory to determine if the cause of death may be related to an infection, which would lend more weight to workup of an organism that might otherwise be considered invasion from normal flora.

Causes For Rejection: Any sample rejections must be approved by the Group Manager, Director of Laboratory Operations, or Laboratory Director after consultation with the OCME

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results:

  • Quantification of organisms.
  • Organisms reported by cellular morphology.
  • Full identification will be performed for significant pathogens, pure culture isolates or a predominating organism in a mixed culture, and at the request of the OCME.

 

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? The microbiology laboratory should be contacted prior to specimen collection for diphtheria, because special media is required for the isolation of these agents.

Collection Container: Dry cotton or polyester tip swab

Optimal Volume: Not Applicable

Collection Guidelines: Use any routine swab collection system but keep swab dry. Do not moisten swab. Silica gel transport packages should be used if transport time might exceed 24 hours.

Storage Container: Not Applicable

Storage Instructions: Room Temperature

Transportation Instructions: Room Temperature

Test Methodology: Conventional culture techniques and 16S Sequencing

Additional Information: It is best to avoid delays in culture inoculation to provide speed and accuracy in the diagnosis.

Causes For Rejection:

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results: Identification of Corynebacterium diphtheriae, Corynebacterium ulcerans, and Corynebacterium pseudotuberculosis. These isolates will be forwarded to the Centers for Disease Control and Prevention (CDC) for toxin testing.

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? No

Collection Container: Sterile Cup

Optimal Volume: Not Applicable

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible so that sample is processed no longer than 48 hours after collection.

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Transport with Cold Packs

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Due to agonal invasion of sterile tissues with skin, fecal or normal respiratory flora, the OCME may be contacted to direct identification of isolates. Patient history information provided by the OCME assists the laboratory to determine if the cause of death may be related to an infection, which would lend more weight to workup of an organism that might otherwise be considered invasion from normal flora.

Causes For Rejection: Any sample rejections must be approved by the Group Manager, Director of Laboratory Operations, or Laboratory Director after consultation with the OCME

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results:

  • Quantification of organisms.
  • Organisms reported by cellular morphology.
  • Full identification will be performed for significant pathogens, pure culture isolates or a predominating organism in a mixed culture, and at the request of the OCME.

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? No

Collection Container: Sterile Cup

Optimal Volume: >1ml

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible so that sample is processed no longer than 48 hours after collection.

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Transport with Cold Packs

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Due to agonal invasion of sterile tissues with skin, fecal or normal respiratory flora, the OCME may be contacted to direct identification of isolates. Patient history information provided by the OCME assists the laboratory to determine if the cause of death may be related to an infection, which would lend more weight to workup of an organism that might otherwise be considered invasion from normal flora.

Causes For Rejection: Any sample rejections must be approved by the Group Manager, Director of Laboratory Operations, or Laboratory Director after consultation with the OCME

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results:

  • Quantification of organisms.
  • Organisms reported by cellular morphology.
  • Full identification will be performed for significant pathogens, pure culture isolates or a predominating organism in a mixed culture, and at the request of the OCME.

Test Description: Cultivation and identification of bacteria from postmortem specimens received from the Office of the Chief Medical Examiner (OCME).

Order Approval Required? No

Collection Container: Swab

Optimal Volume: Not Applicable

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible so that sample is processed no longer than 48 hours after collection.

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Transport with Cold Packs

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Due to agonal invasion of sterile tissues with skin, fecal or normal respiratory flora, the OCME may be contacted to direct identification of isolates. Patient history information provided by the OCME assists the laboratory to determine if the cause of death may be related to an infection, which would lend more weight to workup of an organism that might otherwise be considered invasion from normal flora.

Causes For Rejection: Any sample rejections must be approved by the Group Manager, Director of Laboratory Operations, or Laboratory Director after consultation with the OCME

  • Samples with no name or identifier, mismatched samples and test request forms.
  • Incorrect or broken transport media.
  • Evidence of exposure to temperature extremes during transport.

Possible Results:

  • Quantification of organisms.
  • Organisms reported by cellular morphology.
  • Full identification will be performed for significant pathogens, pure culture isolates or a predominating organism in a mixed culture, and at the request of the OCME.