Virginia Gov Portal Skip to main content
Division of Consolidated Laboratory Services


Laboratory: Immunology/Virology

Test Description: ABO grouping, Rhesus (Rh) type and reverse grouping can be tested together using a microplate. The agglutination reactions are visualized within the microtiter plate. Human red cells possessing antigens will agglutinate in the presence of the antibody directed toward the antigens. Serum antibodies will agglutinate with the manufactured red cells.

Order Approval Required? No

Specimen Type: Coagulated Blood

Collection Container: Red Top Tube or Lavender top

Optimal Volume: 5 ml

Collection Guidelines: Blood should be collected by standard venipuncture technique and stored at 2-8 °C. Blood may be drawn in EDTA or without anticoagulant. A minimum of 2ml of serum/plasma and cells is required.

Storage Container: Samples should be stored in original tubes for 7 days in a 2-8 °C refrigerator.

Storage Instructions: Samples should be stored at 2-8 °C for 7 days after collection.

Transportation Instructions: Ship on cold packs

Test Methodology: Agglutination

Additional Information: Note: Collection tube with serum separator is unacceptable for this testing ** When a Blood group and Type is ordered, an Atypical Antibody test is also performed.

Causes For Rejection:

  • Grossly Hemolyzed
  • Quantity not sufficient for testing
  • Improperly labeled
  • Patient younger than 6 months
  • Patient with agammaglobunemia

Possible Results: A positive, A negative, B positive, B negative, O positive, O negative, AB positive, AB negative, inconclusive/Antibody Screen: positive , negative