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Division of Consolidated Laboratory Services

BRUCELLA MICROAGGLUTINATION TEST

Laboratory: Immunology/Virology

Test Description: This procedure is used for the detection and quantitation of total and Immunoglobulin G (IgG) antibodies to Brucella sp.

Order Approval Required? Yes

Specimen Type: Serum

Collection Container: Red top or serum separator tubes

Optimal Volume: 2mL

Collection Guidelines: Paired acute and convalescent serum specimens obtained at least 14 days apart should be collected, place in 2mL sterile, screw-capped cryovials, and stored at less than or equal to -20 °C both samples can be shipped together. Specimens should be shipped cold or frozen.

Storage Container: Ship in original tube on cold packs or in cryovial frozen.

Storage Instructions: Samples should be stored at -20 °C after testing indefinitely.

Transportation Instructions: Samples should be shipped cold or frozen

Test Methodology: Agglutination

Additional Information: Must have Virginia Department of Health (VDH) and Division of Consolidated Laboratory Services (DCLS) approval to perform test

Causes For Rejection:

  • Hemolyzed specimens
  • Hyperlimic specimens
  • Contaminated serum

Possible Results: Presumptive evidence