CHICKEN WNV/EEE

Laboratory

Immunology/Virology

Description

Qualitative detection of recently generated West Nile virus (WNV) or Eastern Equine Encephalitis (EEE) virus IgY antibodies in serum of infected sentinel chickens. This will provide data needed to determine the incidence of WNV or EEE infection during each Arboviral season.

Specimen Types

• Whole brachial blood
• Serum

Sample Volume / Collection Container

5 mL of whole brachial blood or serum in a serum separator tube (SST).

Collection and Storage Guidelines

Samples should be stored at 2 to 8°C until shipment and must be received within 120 days from collection. If a delay is anticipated (over 120 days from collection), serum must be separated from the clot and stored at -20°C or lower.

Transportation Instructions

Samples should be shipped with frozen cold packs.

Test Methodology

Rapid Microsphere-based Immunofluorescence Assay (MIA)

Causes for Rejection

• Samples that are severely hemolyzed, lipemic, bacterially contaminated, or broken in transit.
• The identification on the submission form and sample(s) do not match.
• Quantity of sample is less than 500 μL.