HUMAN ARBO VIRAL PANEL
Laboratory: Immunology/Virology
Test Description: Immunoglobulin M (IgM) Microsphere Immunofluorescence Assay (MIA ), IgM Enzyme-linked Immunosorbent Assay (ELISA) and Immunoglobulin G (IgG) ELISA provides a useful alternative to immunofluorescence for identification of a serologic response to an Arbovirus.
Order Approval Required? Yes
Collection Container: Sterile Container
Optimal Volume: 2ml
Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum specimen should be collected 2-3 weeks after the acute serum or CSF specimen
Storage Container: Aliquot into Cryovial and freeze indefinitely
Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer
Transportation Instructions: Acute samples should be collected within 8 days of symptom onset
Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (IgM ELISA)
Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test
Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.
Possible Results: IgM: Positive, Negative, Equivocal, Non-specific. No IgG testing for CSF.
Collection Container: Red Top or serum separator tube
Optimal Volume: 5ml
Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum or CSF specimen should be collected 2-3 weeks after the acute serum or CSF specimen.
Storage Container: Aliquot serum into Cryovial and freeze indefinitely
Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer
Transportation Instructions: Acute samples should be collected within 8 days of symptom onset
Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (Immunoglobulin M (IgM) or Immunoglobulin G (IgG) ELISA)
Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test
Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.
Possible Results: IgM: Positive, Negative, Equivocal Non-specific; ; IgG (if performed): Positive, Negative, Equivocal, Nonspecific
Collection Container: Red Top or serum separator tube
Optimal Volume: 2 ml
Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum or CSF specimen should be collected 2-3 weeks after the acute serum or CSF specimen.
Storage Container: Aliquot into Cryovial and freeze indefinitely
Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer
Transportation Instructions: Acute samples should be collected within 8 days of symptom onse
Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (IgM or IgG ELISA)
Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test
Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.
Possible Results: Immunoglobulin M (IgM): Positive, Negative, Equivocal Non-specific; Immunoglobulin G (IgG) (if performed): Positive, Negative, Equivocal, Nonspecific