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Division of Consolidated Laboratory Services

HUMAN ARBO VIRAL PANEL

Laboratory: Immunology/Virology

Test Description: Immunoglobulin M (IgM) Microsphere Immunofluorescence Assay (MIA ), IgM Enzyme-linked Immunosorbent Assay (ELISA) and Immunoglobulin G (IgG) ELISA provides a useful alternative to immunofluorescence for identification of a serologic response to an Arbovirus.

Order Approval Required? Yes

Collection Container: Sterile Container

Optimal Volume: 2ml

Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum specimen should be collected 2-3 weeks after the acute serum or CSF specimen

Storage Container: Aliquot into Cryovial and freeze indefinitely

Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer

Transportation Instructions: Acute samples should be collected within 8 days of symptom onset

Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (IgM ELISA)

Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test

Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.

Possible Results: IgM: Positive, Negative, Equivocal, Non-specific. No IgG testing for CSF.

Collection Container: Red Top or serum separator tube

Optimal Volume: 5ml

Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum or CSF specimen should be collected 2-3 weeks after the acute serum or CSF specimen.

Storage Container: Aliquot serum into Cryovial and freeze indefinitely

Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer

Transportation Instructions: Acute samples should be collected within 8 days of symptom onset

Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (Immunoglobulin M (IgM) or Immunoglobulin G (IgG) ELISA)

Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test

Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.

Possible Results: IgM: Positive, Negative, Equivocal Non-specific; ; IgG (if performed): Positive, Negative, Equivocal, Nonspecific

Collection Container: Red Top or serum separator tube

Optimal Volume: 2 ml

Collection Guidelines: Acute serum or cerebrospinal fluid (CSF) should be collected 0-8 days after onset of symptoms and the convalescent serum or CSF specimen should be collected 2-3 weeks after the acute serum or CSF specimen.

Storage Container: Aliquot into Cryovial and freeze indefinitely

Storage Instructions: Should be kept at 2 to 8 °C. Serum should be stored in a -20 or lower freezer and CSF should be stored in a -70°C freezer

Transportation Instructions: Acute samples should be collected within 8 days of symptom onse

Test Methodology: Immunoglobulin M (IgM) Microsphere Immunoassay (MIA) or Enzyme Linked Immunosorbent Assay (IgM or IgG ELISA)

Additional Information: Must have Division of Consolidated Laboratory Services (DCLS) approval to perform test

Causes For Rejection: Quantity not sufficient for testing, Severely hemolyzed, lipemic, bacterially contaminated, or broken in transit; Not properly labeled.

Possible Results: Immunoglobulin M (IgM): Positive, Negative, Equivocal Non-specific; Immunoglobulin G (IgG) (if performed): Positive, Negative, Equivocal, Nonspecific