INFLUENZA PCR
Laboratory
Molecular Detection and Characterization (MDC)
Description
Qualitative detection and subtyping of Influenza nucleic acid by real-time reverse transcription polymerase chain reaction (RT-PCR).
Specimen Type
Nasopharyngeal swab (Rayon/Dacron) in viral transport media (VTM) or universal transport media (UTM)
Collection Container
Sterile tube (optimally 15mL conical) containing 2-3 mL VTM or UTM
Optimal Volume
3 mL
Collection and Submission Guidelines
- Specimens must be received within 72 hours of collection.
- Instructions: Respiratory Illness Collection and Submission Instructions (55083)
- DCLS Influenza Test Request Form
- DCLS Influenza Testing Reminders
Submitters can request collection kits by emailing DCLS_SSSKits@dgs.virginia.gov.
Storage Instructions
Store specimen at 2-8°C until shipment. If a delay is anticipated (greater than 72 hours after collection), store specimens at ≤-70°C.
Transportation Instructions
Transport specimens on ice packs at 2-8°C. Specimens must be received at DCLS within 72 hours after collection. If a delay is anticipated (greater than 72 hours after collection), store at ≤-70°C and ship at ≤-70°C on dry ice.
Test Methodology:
Real-Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR)
Reference Values
Additional Information:
If infection with a novel or highly pathogenic influenza virus is suspected based on current clinical and epidemiological screening criteria (e.g. travel history), specimens should be collected with appropriate infection control precautions and advanced notice must be provided to DCLS.
- Please refer to the following document for guidance: Highly Pathogenic Avian Influenza Submission Instructions (55328)
Causes For Rejection:
- Incorrect specimen type (swab site or transport media)
- Absence of or inconsistent specimen labeling and identification
- Specimen leaked in transit
- Specimen submission form not properly filled out
- Specimen received in expired transport medium
- Specimen received at the laboratory more than 72 hours after collection
- Specimen with insufficient volume for testing
- Specimen received out of the appropriate temperature range