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Division of Consolidated Laboratory Services

MEASLES IMMUNOGLOBULIN M (IGM) & IMMUNOGLOBULIN G (IGG)

Laboratory: Immunology/Virology

Test Description: The Captia Measles Immunoglobulin G (IgG) Enzyme-linked Immunosorbent Assay (ELISA) is intended for the detection and quantitative determination of IgG antibody to Measles (Rubeola) virus in human sera. Individual serum specimens may be used for the determination of immune status. Paired sera, acute and convalescent, may be used to demonstrate seroconversion or significant rise in antibody as an aid in the diagnosis of recent or current infection. This is an in vitro high complexity test. The CaptiaTM Measles Immunoglobulin M (IgM) Enzyme-linked Immunosorbent Assay (ELISA) is intended for the detection and quantitative determination of IgM antibody to Measles (Rubeola) virus in human sera. Individual serum specimens may be used for the determination of immune status. Paired sera, acute and convalescent, may be used to demonstrate seroconversion or significant rise in antibody as an aid in the diagnosis of recent or current infection. This is an in vitro high complexity test.

Order Approval Required? Yes

Specimen Type: Serum

Collection Container: red top or serum separator tube

Optimal Volume: 2 ml

Collection Guidelines: Blood should be collected in a red top or serum separator tube. Optimal volume of serum is 2mL but the minimal volume of 50uL of serum is acceptable. Acute samples should be collected as soon as possible after onset of symptoms. The second sample (convalescent sample) should be collected 14 to 21 days after the acute sample is collected.

Storage Container: Store serum between 2-8 °C if testing will take place within two days. If specimens are to be kept for longer than 2 days, store at -20 °C or colder.

Storage Instructions: Store specimens at (2-8°C) for 2 days. If the duration of storage exceeds 2 days, store specimens at -20˚C. Avoid repeated freeze-thaw cycles.

Transportation Instructions: Acute samples should be collected as soon as possible after onset of symptoms; the second sample (convalescent sample) should be collected 14 to 21 days after the acute sample is collected.

Test Methodology: ELISA

Additional Information: Must have Virginia Department of Health (VDH) and Division of Consolidated Laboratory Services (DCLS) approval to perform test

Causes For Rejection:

  • Hemolysis, lipemia, or icterus samples are not acceptable
  • Neonate specimens received for IgG testing
  • Improperly labeled specimens
  • Specimen has apparent contamination or particulate matter present
  • Improperly stored specimens

Possible Results: Negative, Positive, Equivocal