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Division of Consolidated Laboratory Services

NEISSERIA GONORRHOEAE (GC) CULTURE

Laboratory: Microbial Reference

Test Description: Isolation and Identification of Neisseria gonorrhoeae (from throat, rectal, cervical, male urethral, other)

Order Approval Required? No

Specimen Type: JEMBEC or Isolate Slant

Collection Container: JEMBEC Transport System or Chocolate Agar Slant

Optimal Volume: Not Applicable

Collection Guidelines: JEMBECs are used for direct collection of throat, rectal, cervical, and male urethral clinical specimens. Detailed collection instructions are provided on DGS-34-117.Neisseria gonorrhoeae isolate slants may be sent for identification or confirmation from any source.

Storage Container: Not Applicable

Storage Instructions: JEMBECs must be delivered to DCLS the same day they are inoculated, or they must be incubated a minimum of 16 hours at 35-37°C before transport.

Transportation Instructions: For JEMBEC specimens, refer to Specimen Submission Policy for Neisseria gonorrhoeae culture screening on DGS-34-117.For isolate slants, transport at Room Temperature. Increased transport time may lead to non-viability.

Test Methodology: Conventional culture techniques, Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) and 16S Sequencing

Additional Information: Kits for the screening of Neisseria gonorrhoeae are provided to State Health Departments by the Division of Consolidated Laboratory Services (DCLS) by calling (804) 648-4480 x 104. Submitters must use materials provided in the kit. Do not interchange materials from other kits.

Causes For Rejection:

  • Specimens with no name or identifier, mismatched specimens and test request forms.
  • Badly broken media or media that has fallen into the top of the plate, deteriorated media
  • Media overgrown with bacteria or fungi, media that has expired prior to inoculation
  • Lack of evidence of CO2 tablet
  • Lack of zip-lock bag or unsealed bag
  • Demonstration of improper collection, incorrect transport media, exposure to temperature extremes
  • Lack of pre-incubation or specimen not received the same day; specimen refrigerated
  • Cultures in route more than 48 hours with only 24 hours pre-incubation.

Possible Results: All Neisseria gonorrhoeae isolates are fully identified using 2 confirmatory methods.