RESPIRATORY PANEL PCR

Laboratory

Molecular Detection and Characterization (MDC)

Description

Qualitative detection of nucleic acid from respiratory pathogens using real-time reverse transcription polymerase chain reaction (RT-PCR).

• Pathogens detected:

  • Adenovirus
  • Coronavirus (229E, HKU1, NL63, OC43)
  • SARS-CoV-2
  • Human Metapneumovirus
  • Human Rhinovirus/Enterovirus
  • Influenza A
  • Influenza A H1
  • Influenza A H1-2009
  • Influenza A H3
  • Influenza B
  • Parainfluenza 1
  • Parainfluenza 2
  • Parainfluenza 3
  • Parainfluenza 4
  • Respiratory Syncytial Virus A
  • Respiratory Syncytial Virus B
  • Chlamydia pneumoniae
  • Mycoplasma pneumoniae

Specimen Type

Nasopharyngeal swab (Rayon/Dacron) in viral transport media (VTM)

Collection Container

Sterile tube (optimally 15mL conical) containing 2-3mL of VTM

Optimal Volume

3mL

Collection and Submission Guidelines

• Instructions: Respiratory Illness Collection and Submission Instructions (55083)
• DCLS Test Request Form

Submitters can request collection kits by emailing DCLS_SSSKits@dgs.virginia.gov.

Storage Instructions

Store specimens at 2-8°C.

Transportation Instructions

Transport specimens on frozen cold packs at 2-8°C.

Test Methodology

Real-Time Polymerase Chain Reaction (RT-PCR)

Reference Values

Causes For Rejection:

• Incorrect specimen type (swab site or transport media)
• Absence of or inconsistent specimen labeling and identification
• Specimen leaked in transit
• Specimen submission form not properly filled out
• Specimen received in expired transport medium
• Specimen received outside of holding time
• Specimen with insufficient volume for testing
• Specimen received out of the appropriate temperature range