SARS-COV-2 PCR
Laboratory
Molecular Detection and Characterization (MDC)
Description
Qualitative detection of SARS-CoV-2 nucleic acid by real-time reverse transcription polymerase chain reaction (rRT-PCR)
Specimen Type
Nasopharyngeal swab (Rayon/Dacron) in viral transport media (VTM)
Optimal Volume
3 mL
Collection and Submission Guidelines
- Specimens must be received within 72 hours of collection.
- Instructions: Respiratory Illness Collection and Submission Instructions (55083)
- DCLS Test Request Form
Submitters can request collection kits by emailing DCLS_SSSKits@dgs.virginia.gov.
Storage Instructions
Store specimens at 2-8°C .
Transportation Instructions
Transport specimens on frozen cold packs at 2-8°C.
Test Methodology
Real-Time Reverse Transcription Polymerase Chain Reaction (rRT-PCR)
Reference Values
Population
Units
Reference Range
All Populations
(none)
Negative
Causes For Rejection
- Incorrect specimen type (swab site or transport media)
- Absence of or inconsistent specimen labeling and identification
- Specimen leaked in transit
- Specimen submission form not properly filled out
- Specimen received in expired transport medium
- Specimen received at the laboratory more than 72 hours after collection
- Specimen with insufficient volume for testing
- Specimen received out of the appropriate temperature range