Virginia Gov Portal Skip to main content
Division of Consolidated Laboratory Services

SYPHILIS RAPID PLASMA REAGIN (RPR)

Laboratory: Immunology/Virology

Test Description: The Rapid Plasma Reagin (RPR) Card Test (Arlington Scientific Inc.) is a qualitative and semi-quantitative flocculation test for the detection of reagin antibodies in human serum and plasma as a screening test in syphilis serology.

Order Approval Required? No

Specimen Type: Serum

Collection Container: Red Top or serum separator tube

Optimal Volume: 7mL

Collection Guidelines: The optimal amount of specimen is 7ml of coagulated blood in a red top or serum separator tube. 1 ml of coagulated blood in a red top tube or 500uL of sera is acceptable.

Storage Container: Samples are refrigerated for 5 days in original specimen tubes. They may be aliquotted into a cryovial and are frozen in -20 °C freezer.

Storage Instructions: Samples should be stored at 2-8 °C for 5 days after collection. Serum may be aliquotted and stored at 2-8 °C for shipping or frozen at -20°C or colder.

Transportation Instructions: Ship on cold packs

Test Methodology: Flocculation by Rapid Plasma Reagin (RPR) Screening Test

Additional Information:

Causes For Rejection:

  • Identification on the form and tube does not match.
  • Specimen is grossly hemolyzed or lipemic.
  • Specimen has apparent contamination or particulate matter present.
  • Received insufficient quantity of sera/plasma for testing
  • Specimens were maintained under improper storage conditions or out of holding time

Possible Results: Reactive (titers included), Non-reactive