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Division of Consolidated Laboratory Services

TUBERCULOSIS CLINICAL SPECIMEN

Laboratory: Microbial Reference

Test Description: Process clinical specimens for the recovery and identification of Mycobacteria spp. Perform Antimicrobial susceptibilities performed on initial Mycobacterium tuberculosis

Laboratory: Microbial Reference

Order Approval Required? Fee for Service Agreement for non - Virginia Department of Health (VDH) submitters

Collection Container: Sulphated polysaccharides (SPS) or Heparin tube

Optimal Volume: Maximum10 ml

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible, preferentially within 24 hours of collection

Storage Container: Not Applicable

Storage Instructions: Room Temperature

Transportation Instructions: Room Temperature

Test Methodology: Conventional culture techniques and Molecular Methods

Additional Information: Kits for collection of clinical specimens are provided to State Health Departments by the Division of Consolidated Laboratory Services (DCLS) by calling (804) 648-4480 x 104.

Causes For Rejection: Specimen with no name or identifier, mismatched sample and test request, leaked out during transit, not submitted in Sulphated polysaccharides (SPS) or heparin.

Possible Results: Identification of Mycobacteria spp. Drug susceptibility testing (DST) results on first time Mycobacterium tuberculosis isolates. No Acid Fast Bacilli Isolated in Culture.

Test Description: Process clinical specimens for the recovery and identification of Mycobacteria spp. Perform Antimicrobial susceptibilities performed on initial Mycobacterium tuberculosis

Laboratory: Microbial Reference

Order Approval Required? Fee for Service Agreement for non-Virginia Department of Health submitters

Collection Container: Sterile Container

Optimal Volume: Maximum10 ml

Collection Guidelines: Specimen should be transported to the laboratory as soon as possible, preferentially within 24 hours of collection

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Transport with Cold Packs

Test Methodology: N-Acetyl-L-Cysteine (NALC) specimen processing; Fluorochrome smears; Conventional and Molecular Methods for identification

Additional Information: Kits for collection of clinical specimens are provided to State Health Departments by the Division of Consolidated Laboratory Services (DCLS) by calling (804) 648-4480 x 104.

Causes For Rejection: Specimen with no name or identifier, leaked out during transit, visibly contaminated with foreign substance or too old, greater than 7 days from collection.

Possible Results: Identification of Mycobacteria spp. Drug susceptibility testing (DST) results on first time Mycobacterium tuberculosis isolates. No Acid Fast Bacilli Isolated in Culture.

Test Description: Process clinical specimens for the recovery and identification of Mycobacteria spp. Perform Antimicrobial susceptibilities performed on initial Mycobacterium tuberculosis

Laboratory: Microbial Reference

Order Approval Required? (Note: For non-Virginia Department of Health Submitters there is an associated fee for this service. Please contact Microbial Reference Group Manager 804-648-4480 ext 210 for more information.) Fee for Service Agreement for non-Virginia Department of Health submitters

Collection Container: Sterile Container

Optimal Volume: Maximum 10 ml

Collection Guidelines: First morning sputum. Specimen should be transported to the laboratory as soon as possible, preferentially within 24 hours of collection

Storage Container: Not Applicable

Storage Instructions: Store at 2-8°C

Transportation Instructions: Transport with Cold Packs

Test Methodology: N-Acetyl-L-Cysteine (NALC) specimen processing; Direct Nucleic Acid Amplification Test (NAAT) for Mycobacterium tuberculosis complex; Fluorochrome smears; Conventional and Molecular Methods for identification

Additional Information: Kits for collection of clinical specimens are provided to State Health Departments by the Division of Consolidated Laboratory Services (DCLS) by calling (804) 648-4480 x 104.

Causes For Rejection: Specimen with no name or identifier, leaked out during transit, visibly contaminated with foreign substance or too old, greater than 7 days from collection.

Possible Results: Identification of Mycobacteria spp. Drug susceptibility testing (DST) results on first time Mycobacterium tuberculosis isolates. No Acid Fast Bacilli Isolated in Culture.